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sequences encoding mruby2  (Addgene inc)


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    Addgene inc sequences encoding mruby2
    Asynchronous normalized Ca 2+ signal responses of the root hair (RH) compared to non-root hair (NRH) bearing epidermal cells showing distinct signatures comprising waves and spikes under low (0.25 mM) and high (5 mM) NO 3 - treatment. (A) The kinetics of apparent Ca 2+ signal represented through the GFP fluorescence intensity responding to low NO 3 - treatment. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with <t>mRuby2</t> RFP background and captures the response 90 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 190 seconds for all RH sections (RH-base, RH-mid and RH-tip) while NRH cell had peak normalized Ca 2+ signal intensity was around 240 seconds after low NO 3 - treatment. (B) The kinetics of apparent Ca 2+ signal responding to high NO 3 - treatment for the selected root sections. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 110 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 120 seconds for RH-tip, and close to 140 seconds for RH-mid and RH-base sections.
    Sequences Encoding Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequences encoding mruby2/product/Addgene inc
    Average 95 stars, based on 277 article reviews
    sequences encoding mruby2 - by Bioz Stars, 2026-04
    95/100 stars

    Images

    1) Product Images from "High and low exogenous nitrate concentrations produce distinct calcium signatures in Arabidopsis roots"

    Article Title: High and low exogenous nitrate concentrations produce distinct calcium signatures in Arabidopsis roots

    Journal: bioRxiv

    doi: 10.1101/2025.03.03.641058

    Asynchronous normalized Ca 2+ signal responses of the root hair (RH) compared to non-root hair (NRH) bearing epidermal cells showing distinct signatures comprising waves and spikes under low (0.25 mM) and high (5 mM) NO 3 - treatment. (A) The kinetics of apparent Ca 2+ signal represented through the GFP fluorescence intensity responding to low NO 3 - treatment. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 90 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 190 seconds for all RH sections (RH-base, RH-mid and RH-tip) while NRH cell had peak normalized Ca 2+ signal intensity was around 240 seconds after low NO 3 - treatment. (B) The kinetics of apparent Ca 2+ signal responding to high NO 3 - treatment for the selected root sections. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 110 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 120 seconds for RH-tip, and close to 140 seconds for RH-mid and RH-base sections.
    Figure Legend Snippet: Asynchronous normalized Ca 2+ signal responses of the root hair (RH) compared to non-root hair (NRH) bearing epidermal cells showing distinct signatures comprising waves and spikes under low (0.25 mM) and high (5 mM) NO 3 - treatment. (A) The kinetics of apparent Ca 2+ signal represented through the GFP fluorescence intensity responding to low NO 3 - treatment. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 90 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 190 seconds for all RH sections (RH-base, RH-mid and RH-tip) while NRH cell had peak normalized Ca 2+ signal intensity was around 240 seconds after low NO 3 - treatment. (B) The kinetics of apparent Ca 2+ signal responding to high NO 3 - treatment for the selected root sections. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 110 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 120 seconds for RH-tip, and close to 140 seconds for RH-mid and RH-base sections.

    Techniques Used: Fluorescence

    Asynchronous normalized Ca 2+ signal in the root sections in response to the low NO 3 - treatment. A representative root section for the low NO 3 - treated seedling is shown with the normalized Ca 2+ signal, where section S1 was distal to the root tip while S4 was proximal to the root tip. The root tissue imaged above shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 120 seconds after low NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 90 seconds for sections S1 and S2, while the peak intensity of the normalized Ca 2+ signal was close to 125 seconds for S3 and 60 seconds for S4.
    Figure Legend Snippet: Asynchronous normalized Ca 2+ signal in the root sections in response to the low NO 3 - treatment. A representative root section for the low NO 3 - treated seedling is shown with the normalized Ca 2+ signal, where section S1 was distal to the root tip while S4 was proximal to the root tip. The root tissue imaged above shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 120 seconds after low NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 90 seconds for sections S1 and S2, while the peak intensity of the normalized Ca 2+ signal was close to 125 seconds for S3 and 60 seconds for S4.

    Techniques Used: Fluorescence



    Similar Products

    sequences encoding mruby2  (Addgene inc)
    95
    Addgene inc sequences encoding mruby2
    Asynchronous normalized Ca 2+ signal responses of the root hair (RH) compared to non-root hair (NRH) bearing epidermal cells showing distinct signatures comprising waves and spikes under low (0.25 mM) and high (5 mM) NO 3 - treatment. (A) The kinetics of apparent Ca 2+ signal represented through the GFP fluorescence intensity responding to low NO 3 - treatment. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with <t>mRuby2</t> RFP background and captures the response 90 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 190 seconds for all RH sections (RH-base, RH-mid and RH-tip) while NRH cell had peak normalized Ca 2+ signal intensity was around 240 seconds after low NO 3 - treatment. (B) The kinetics of apparent Ca 2+ signal responding to high NO 3 - treatment for the selected root sections. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 110 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 120 seconds for RH-tip, and close to 140 seconds for RH-mid and RH-base sections.
    Sequences Encoding Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequences encoding mruby2/product/Addgene inc
    Average 95 stars, based on 1 article reviews
    sequences encoding mruby2 - by Bioz Stars, 2026-04
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Asynchronous normalized Ca 2+ signal responses of the root hair (RH) compared to non-root hair (NRH) bearing epidermal cells showing distinct signatures comprising waves and spikes under low (0.25 mM) and high (5 mM) NO 3 - treatment. (A) The kinetics of apparent Ca 2+ signal represented through the GFP fluorescence intensity responding to low NO 3 - treatment. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 90 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 190 seconds for all RH sections (RH-base, RH-mid and RH-tip) while NRH cell had peak normalized Ca 2+ signal intensity was around 240 seconds after low NO 3 - treatment. (B) The kinetics of apparent Ca 2+ signal responding to high NO 3 - treatment for the selected root sections. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 110 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 120 seconds for RH-tip, and close to 140 seconds for RH-mid and RH-base sections.

    Journal: bioRxiv

    Article Title: High and low exogenous nitrate concentrations produce distinct calcium signatures in Arabidopsis roots

    doi: 10.1101/2025.03.03.641058

    Figure Lengend Snippet: Asynchronous normalized Ca 2+ signal responses of the root hair (RH) compared to non-root hair (NRH) bearing epidermal cells showing distinct signatures comprising waves and spikes under low (0.25 mM) and high (5 mM) NO 3 - treatment. (A) The kinetics of apparent Ca 2+ signal represented through the GFP fluorescence intensity responding to low NO 3 - treatment. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 90 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 190 seconds for all RH sections (RH-base, RH-mid and RH-tip) while NRH cell had peak normalized Ca 2+ signal intensity was around 240 seconds after low NO 3 - treatment. (B) The kinetics of apparent Ca 2+ signal responding to high NO 3 - treatment for the selected root sections. The root tissue imaged shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 110 seconds after the NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 120 seconds for RH-tip, and close to 140 seconds for RH-mid and RH-base sections.

    Article Snippet: Sequences encoding mRuby2 (#40260, ( ) and GCaMP6s (#40753, ( ) were obtained from Addgene (Watertown, MA).

    Techniques: Fluorescence

    Asynchronous normalized Ca 2+ signal in the root sections in response to the low NO 3 - treatment. A representative root section for the low NO 3 - treated seedling is shown with the normalized Ca 2+ signal, where section S1 was distal to the root tip while S4 was proximal to the root tip. The root tissue imaged above shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 120 seconds after low NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 90 seconds for sections S1 and S2, while the peak intensity of the normalized Ca 2+ signal was close to 125 seconds for S3 and 60 seconds for S4.

    Journal: bioRxiv

    Article Title: High and low exogenous nitrate concentrations produce distinct calcium signatures in Arabidopsis roots

    doi: 10.1101/2025.03.03.641058

    Figure Lengend Snippet: Asynchronous normalized Ca 2+ signal in the root sections in response to the low NO 3 - treatment. A representative root section for the low NO 3 - treated seedling is shown with the normalized Ca 2+ signal, where section S1 was distal to the root tip while S4 was proximal to the root tip. The root tissue imaged above shows the apparent Ca 2+ signal through GFP fluorescence with mRuby2 RFP background and captures the response 120 seconds after low NO 3 - treatment. The peak normalized Ca 2+ signal intensity was captured around 90 seconds for sections S1 and S2, while the peak intensity of the normalized Ca 2+ signal was close to 125 seconds for S3 and 60 seconds for S4.

    Article Snippet: Sequences encoding mRuby2 (#40260, ( ) and GCaMP6s (#40753, ( ) were obtained from Addgene (Watertown, MA).

    Techniques: Fluorescence